Date of Award

January 2018

Degree Type

Open Access Thesis

Document Type

Master Thesis

Degree Name

Master of Science (MS)



First Advisor

Donghui Quan

Department Affiliation


Second Advisor

Stephen C. Richter

Department Affiliation

Biological Sciences

Third Advisor

Pei Gao

Department Affiliation



Atrazine is the most widely used herbicides in USA, but its negative impact on aquatic ecosystem cannot be over-looked. Atrazine was traditionally determined through liquid/gas chromatography or IR spectroscopy. However, these methods are either time-consuming or costly, so fluorescence spectroscopy is investigated as a more cost and time efficient alternative. Literature value of the optimal wavelength of atrazine was found to be 350 nm emission. Experimental value for emission scanned of atrazine resulted in an optimal wavelength of 363 nm. Known and unknown concentrations of atrazine will be analyzed using fluorescence spectrophotometer to develop a calibration curve.

The calibration concentration curve developed would be used in the future as an analytical technique to determine the concentrations of atrazine in real local water samples collected by the Biology Department at EKU.