The objective of this study was to establish an assay for triclosan resistance in bacteria found in the environment. This assay was then used to test bacteria from several points along the Kentucky River and other local bodies of water. Triclosan is a hydrophobic molecule that requires another compound to increase solubility in water. Numerous trials were involved in developing the assay. Sodium lauryl sulfate (SLS) was determined as the most useful solubilizing agent. Media was then made with tryptic soy agar (TSA) supplemented with 0.05% SLS and 0.1% triclosan, and 0.05% SLS alone as a control. SLS was found to inhibit bacterial growth. Resistance to triclosan was also observed. Bacteria showing evidence of resistance were tested against antibiotics. Co-resistance for triclosan and β-lactam antibiotics was observed.
Semester/Year of Award
William J. Staddon
Restricted Access Thesis
P'Pool, Jessie, "The Development and Testing of an Assay for Triclosan Resistance in Environmental Samples" (2013). Honors Theses. 76.