Project Title

Genetic Investigation of antibiotic resistance in Streptococcus pyogenes

Presenter Hometown

Richmond

Major

Biomedical Sciences

Department

Biological Sciences

Degree

Undergraduate

Mentor

Marcia Pierce

Mentor Department

Biological Sciences

Abstract

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a gram-positive bacterium which may cause noninvasive and invasive diseases as well as immune mediated squelae such as Pharyngitis, Scarlet Fever, Type II Necrotizing Fasciitis, Rheumatic Fever, and Post-Streptococcal Glomerulonephritis. Upsurges of S. pyogenes infection incidence is associated with the introduction of novel strains within a population made especially concerning by a resurgence of reported GAS disease in US, Europe and elsewhere the past few decades. The lack of S. pyogenes vaccine, the acquisition of penicillin resistance, and the resurgence suggesting a vast array of new, potentially resistant, strains highlight the importance identifying the mechanism of resistance between S. pyogenes strains through genomic study. The objective of this research will be to determine mechanisms of antibiotic resistance in the GAS isolates and analyze their genomic information. Verified strains of Group A Streptococcus will be tested for antibiotic resistance using Kirby-Baur protocol. The genomic DNA of the isolates will be purified using the ChargeSwitch® gDNA Mini Bacteria Kit which uses ionizable magnetic beads. PCR amplification will then be used to amplify the DNA with erm B, ermTR, and mefA gene primers. Additional Strains of S. pyogenes will be obtained. Tested strains will be sequenced for pan-genome project analysis.

Presentation format

Poster

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Genetic Investigation of antibiotic resistance in Streptococcus pyogenes

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a gram-positive bacterium which may cause noninvasive and invasive diseases as well as immune mediated squelae such as Pharyngitis, Scarlet Fever, Type II Necrotizing Fasciitis, Rheumatic Fever, and Post-Streptococcal Glomerulonephritis. Upsurges of S. pyogenes infection incidence is associated with the introduction of novel strains within a population made especially concerning by a resurgence of reported GAS disease in US, Europe and elsewhere the past few decades. The lack of S. pyogenes vaccine, the acquisition of penicillin resistance, and the resurgence suggesting a vast array of new, potentially resistant, strains highlight the importance identifying the mechanism of resistance between S. pyogenes strains through genomic study. The objective of this research will be to determine mechanisms of antibiotic resistance in the GAS isolates and analyze their genomic information. Verified strains of Group A Streptococcus will be tested for antibiotic resistance using Kirby-Baur protocol. The genomic DNA of the isolates will be purified using the ChargeSwitch® gDNA Mini Bacteria Kit which uses ionizable magnetic beads. PCR amplification will then be used to amplify the DNA with erm B, ermTR, and mefA gene primers. Additional Strains of S. pyogenes will be obtained. Tested strains will be sequenced for pan-genome project analysis.