Project Title

Isolating Novel Phages Using Mycobacterium smegmatis

Presenter Hometown

Richmond, Kentucky

Major

Biomedical Sciences

Department

Biological Sciences

Degree

Undergraduate

Mentor

Marcia Pierce

Mentor Department

Biological Sciences

Abstract

Bacteriophages are viruses that infect bacterial hosts. Interest in bacteriophage research has grown as a future treatment option for human bacterial diseases, which could be used to avert the mounting crisis of antibiotic resistance. The presented project entitled Isolating Novel Phages Using Mycobacterium smegmatis aims to identify an individual phage possessing unique genetic information that can potentially be useful in further biomedical research. The process begins with the collection of a soil sample which is then enriched and used to create a filtrate. This filtrate was then purified through a series of dilutions, leading to the isolation of an individual phage. Once isolated, through the use of bioinformatics, the single phages genomic, physical, and molecular properties were deduced. This unique information is then characterized and placed into a singular database. This database can be utilized by researchers in current studies combating antibiotic resistance.

Presentation format

Poster

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Isolating Novel Phages Using Mycobacterium smegmatis

Bacteriophages are viruses that infect bacterial hosts. Interest in bacteriophage research has grown as a future treatment option for human bacterial diseases, which could be used to avert the mounting crisis of antibiotic resistance. The presented project entitled Isolating Novel Phages Using Mycobacterium smegmatis aims to identify an individual phage possessing unique genetic information that can potentially be useful in further biomedical research. The process begins with the collection of a soil sample which is then enriched and used to create a filtrate. This filtrate was then purified through a series of dilutions, leading to the isolation of an individual phage. Once isolated, through the use of bioinformatics, the single phages genomic, physical, and molecular properties were deduced. This unique information is then characterized and placed into a singular database. This database can be utilized by researchers in current studies combating antibiotic resistance.