EKU Faculty and Staff Scholarship
 

Title

Neuroproteomic Study of Nitrated Proteins in Moderate Traumatic Brain Injured Rats Treated with Gamma Glutamyl Cysteine Ethyl Ester Administration Post Injury: Insight nto the Role of Glutathione Elevation in Nitrosative Stress

Department

Chemistry

Document Type

Article

Publication Date

10-2016

Abstract

Purpose

The aims of this study are to establish a time point to determine the most beneficial time to administer GCEE post incident to reduce oxidative damage and second, by using redox proteomics, to determine if GCEE can readily suppress 3-NT modification in TBI animals.

Experimental design

By using a moderate traumatic brain injury model with Wistar rats, it is hypothesized that the role of 3-nitrotyrosine (3-NT) formation as an intermediate will predict the involvement of protein nitration/nitrosation and oxidative damage in the brain.

Results

In this experiment, the levels of protein carbonyls, 4-hydroxynonenal, and 3-nitrotyrosine were significantly elevated in TBI injured, saline treated rats compared with those who sustained an injury and were treated with 150 mg/kg of the glutathione mimetic, GCEE.

Conclusion and clinical relevance

Determining the existence of elevated 3-NT levels provides insight into the relationship between the protein nitration/nitrosation and the oxidative damage, which can determine the pathogenesis and progression of specific neurological diseases.

Journal Title

Proteomics--Clinical Applications

Journal Volume

10

Journal Issue

12

Article Starting Page

1218

Article Ending Page

1224