Henderson, M., Rice, B., Sebastian, A., Sullivan, P. G., King, C., Robinson, R. A.S. and Reed, T. T. (2016), Neuroproteomic study of nitrated proteins in moderate traumatic brain injured rats treated with gamma glutamyl cysteine ethyl ester administration post injury: Insight into the role of glutathione elevation in nitrosative stress. Prot. Clin. Appl., 10: 1218–1224. doi:10.1002/prca.201600004
Neuroproteomic Study of Nitrated Proteins in Moderate Traumatic Brain Injured Rats Treated with Gamma Glutamyl Cysteine Ethyl Ester Administration Post Injury: Insight nto the Role of Glutathione Elevation in Nitrosative Stress
The aims of this study are to establish a time point to determine the most beneficial time to administer GCEE post incident to reduce oxidative damage and second, by using redox proteomics, to determine if GCEE can readily suppress 3-NT modification in TBI animals.
By using a moderate traumatic brain injury model with Wistar rats, it is hypothesized that the role of 3-nitrotyrosine (3-NT) formation as an intermediate will predict the involvement of protein nitration/nitrosation and oxidative damage in the brain.
In this experiment, the levels of protein carbonyls, 4-hydroxynonenal, and 3-nitrotyrosine were significantly elevated in TBI injured, saline treated rats compared with those who sustained an injury and were treated with 150 mg/kg of the glutathione mimetic, GCEE.
Conclusion and clinical relevance
Determining the existence of elevated 3-NT levels provides insight into the relationship between the protein nitration/nitrosation and the oxidative damage, which can determine the pathogenesis and progression of specific neurological diseases.
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