Date of Award


Degree Type

Open Access Thesis

Document Type

Master Thesis

Degree Name

Master of Science (MS)


Biological Sciences

First Advisor

Marcia M. Pierce

Department Affiliation

Biological Sciences

Second Advisor

Oliver R. Oakley

Department Affiliation

Biological Sciences

Third Advisor

Lindsay E. Calderon

Department Affiliation

Biological Sciences


Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical use of this pathogen has revealed that the intercellular mechanisms can be exploited to produce a cancer vaccine. Currently L. monocytogenes is being studied as a viable candidate for various other cancer vaccines and therapies. The purpose of this study is to reduce the downstream virulence factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.