Date of Award
2019
Degree Type
Open Access Thesis
Document Type
Master Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
First Advisor
Marcia M. Pierce
Department Affiliation
Biological Sciences
Second Advisor
Oliver R. Oakley
Department Affiliation
Biological Sciences
Third Advisor
Lindsay E. Calderon
Department Affiliation
Biological Sciences
Abstract
Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical use of this pathogen has revealed that the intercellular mechanisms can be exploited to produce a cancer vaccine. Currently L. monocytogenes is being studied as a viable candidate for various other cancer vaccines and therapies. The purpose of this study is to reduce the downstream virulence factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.
Copyright
Copyright 2019 Robert Jackson
Recommended Citation
Jackson, Robert, "Knockout of the trans-acting riboswitches in Listeria monocytogenes to disrupt downstream virulence factors" (2019). Online Theses and Dissertations. 610.
https://encompass.eku.edu/etd/610
