The objective of our research is to develop a viable analysis method capable of determining unknown atrazine concentrations. Traditional instrumentation methods can damage or cause expenditure of samples and are time consuming to use; fluorescence spectroscopy is a relatively cheap, fast, and non-destructive method for concentration analysis. Standard atrazine dilutions are made and fluorescence emission spectra are used to develop a calibration curve. Unknown atrazine samples are analyzed using standards. The results will allow the EKU Biology Department to analyze samples of unknown concentration from local streams and ponds.