Date of Award
January 2016
Degree Type
Open Access Thesis
Document Type
Master Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
First Advisor
Tanea T. Reed
Department Affiliation
Biological Sciences
Second Advisor
Lindsay E. Calderon
Department Affiliation
Biological Sciences
Third Advisor
Marcia M. Pierce
Department Affiliation
Biological Sciences
Abstract
The brain is one of the most important organs in the body. It functions as a control center by regulating and coordinating actions and reactions, which is facilitated via signal transduction pathways. Its function is primarily dependent upon sufficient supply of glucose for energy metabolism. The dysfunction of the brain resulting from an external force is known as traumatic brain injury (TBI). Symptoms range from physical to psychological and effects can be mild, moderate, or severe depending on the extent of injury. TBI is associated with oxidative damage, the overproduction of reactive oxygen/nitrogen species. Reduced energy metabolism is a consequence of traumatic brain injury, while reduced purine salvage is associated with deficient cell signaling. Previous studies have demonstrated that the administration of gamma-glutamylcysteine ethyl ester (GCEE) following TBI has protective effects against protein nitration. This study investigates the enzymatic activity of energy related and salvage related enzymes, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, lactate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, cytochrome C oxidase, ATP synthase, and hypoxanthine-guanine phosphoribosyltransferase, that have been identified as excessively nitrated following the administration of GCEE post-TBI. Adult male Wistar rats were divided equally into three groups: sham, saline, and GCEE. Rats in all groups (except sham) were subjected to a craniotomy and a moderate TBI via cortical contusion. Post-TBI rats in the saline group received an administration of saline (150mg/kg), and rats in the GCEE treatment group received an administration of GCEE (same dosage). Upon sacrifice, brains were harvested and enzymatic activity was indirectly measured spectrophotometrically. Data demonstrates that the administration of GCEE following brain trauma increases enzymatic activity. Our results are promising and indicate potential therapeutic strategies to restore energy and salvage related enzymatic activity in the brain post-TBI.
Copyright
Copyright 2016 Brittany Rice
Recommended Citation
Rice, Brittany, "Enzyme Analysis of Oxidatively Modified Proteins Post-TBI" (2016). Online Theses and Dissertations. 417.
https://encompass.eku.edu/etd/417