Peptide Tyrosine Arginine, a Potent Immunomodulatory Peptide Isolated and Structurally Characterized from the Skin Secretions of the Dusky Gopher Frog, Rana sevosa
Author ORCID Identifier
Stephen Richterhttps://orcid.org/0000-0001-6646-2484
Department
Biological Sciences
Document Type
Article
Publication Date
5-1-2005
Abstract
An octadecapeptide was isolated from the skin secretions of the dusky gopher frog (Rana sevosa) on the basis of histamine release from rat peritoneal mast cells. This peptide was purified to homogeneity by HPLC and found to have the following primary structure, YLKGCWTKSYPPKPCFSR, using both Edman degradation chemistry and peptide sequencing using high-resolution mass spectrometry (Q-TOF MS). The peptide, named peptide Tyrosine Arginine (pYR) shares 77.8% homology with peptide Leucine Arginine (pLR). The effects of the natural amidated peptide, non-amidated peptide and C-loop region of pYR on granulopoiesis and neutrophil apoptosis were investigated. All three analogues inhibited the early development of granulocyte macrophage colonies from bone marrow stem cells but did not induce apoptosis of the end stage granulocytes, the mature neutrophil. Thus, pYR is a novel member of an important and emerging new class of amphibian peptides with hemopoietic actions.
Recommended Citation
Graham, Ciaren; Irvine, Alexandra E.; McClean, Stephen; Richter, Stephen C.; Flatt, Peter R.; and Shaw, Chris, "Peptide Tyrosine Arginine, a Potent Immunomodulatory Peptide Isolated and Structurally Characterized from the Skin Secretions of the Dusky Gopher Frog, Rana sevosa" (2005). EKU Faculty and Staff Scholarship. 665.
https://encompass.eku.edu/fs_research/665
Journal Title
Peptides