Development and Characterization of Polymorphic Microsatellite DNA loci for the Endangered Dusky Gopher Frog, Rana sevosa, and Two Closely Related Species, Rana capito and Rana areolata

Authors

Stephen C. Richter, Eastern Kentucky UniversityFollow
Richard E. Broughton

Author ORCID Identifier

Stephen Richterhttps://orcid.org/0000-0001-6646-2484

Department

Biological Sciences

Document Type

Article

Publication Date

6-1-2005

Abstract

A microsatellite library was developed using genomic DNA of the endangered dusky gopher frog, Rana sevosa. Polymerase chain reaction (PCR) primers and conditions are presented for R. sevosa (eight loci) and two sister taxa — other gopher frogs, Rana capito (seven loci) and crawfish frogs, Rana areolata (three loci). Polymorphism of each microsatellite locus was evaluated for each species. All loci have moderate to high genetic variation in terms of allelic richness (four to 10 alleles per locus), observed heterozygosity (0.595–0.946), and expected heterozygosity (0.531–0.856).

Recommended Citation

Richter, Stephen C. and Broughton, Richard E., "Development and Characterization of Polymorphic Microsatellite DNA loci for the Endangered Dusky Gopher Frog, Rana sevosa, and Two Closely Related Species, Rana capito and Rana areolata" (2005). EKU Faculty and Staff Scholarship. 666.
https://encompass.eku.edu/fs_research/666

Journal Title

Molecular Ecology Notes