The Effects of Gamma Glutamyl Cysteinyl Ethyl Ester on Oxidative Stress Biomarkers in Traumatically Injured Rats

Author

Jenna Shoemaker, Eastern Kentucky UniversityFollow

Abstract

Primary injury is the original point where the impact force affects axonal tissue. Secondary injury is often the main complication in patients suffering from traumatic brain injury (TBI). Consequences of secondary injuries include oxidative stress, DNA damage, and protein oxidation, which can impact overall brain function (Moppett). Gamma-glutamyl cysteinyl ethyl ester (GCEE), a glutathione mimetic, has been used as an antioxidant to reduce oxidative stress. This study aims to determine if GCEE is neuroprotective against moderate TBI at extended time points. Results from this work will provide insight for determining the best extended time point to administer treatment. This study aims to compare the amount of carbonylated proteins in rats given a moderate TBI and treated with GCEE within 90 minutes and 120 minutes of the injury

Quantification of excess carbonylated proteins was measured using a Bio-Rad slot blot apparatus and the methods used by Reed (Reed, 2016). The resulting membrane was analyzed. Results showed that the samples treated with GCEE at 90 minutes had less protein oxidation compared to saline at 90 minutes. However, GCEE administered at 120 minutes had protein oxidation levels like samples treated with saline at 120 minutes.

Semester/Year of Award

Spring 5-7-2024

Mentor

Tanea T. Reed

Mentor Department Affiliation

Chemistry

Access Options

Open Access Thesis

Degree Name

Honors Scholars

Degree Level

Bachelors

Department

Chemistry

Recommended Citation

Shoemaker, Jenna, "The Effects of Gamma Glutamyl Cysteinyl Ethyl Ester on Oxidative Stress Biomarkers in Traumatically Injured Rats" (2024). Honors Theses. 1046.
https://encompass.eku.edu/honors_theses/1046