Abstract
The objective of this study was to establish an assay for triclosan resistance in bacteria found in the environment. This assay was then used to test bacteria from several points along the Kentucky River and other local bodies of water. Triclosan is a hydrophobic molecule that requires another compound to increase solubility in water. Numerous trials were involved in developing the assay. Sodium lauryl sulfate (SLS) was determined as the most useful solubilizing agent. Media was then made with tryptic soy agar (TSA) supplemented with 0.05% SLS and 0.1% triclosan, and 0.05% SLS alone as a control. SLS was found to inhibit bacterial growth. Resistance to triclosan was also observed. Bacteria showing evidence of resistance were tested against antibiotics. Co-resistance for triclosan and β-lactam antibiotics was observed.
Semester/Year of Award
Spring 2013
Mentor
William J. Staddon
Mentor Department Affiliation
Biological Sciences
Access Options
Restricted Access Thesis
Document Type
Bachelor Thesis
Degree Name
Honors Scholars
Degree Level
Bachelor's
Department
Biological Sciences
Recommended Citation
P'Pool, Jessie, "The Development and Testing of an Assay for Triclosan Resistance in Environmental Samples" (2013). Honors Theses. 76.
https://encompass.eku.edu/honors_theses/76