Isolating Novel Phages Using Mycobacterium smegmatis
Presenter Hometown
Richmond, Kentucky
Major
Biomedical Sciences
Department
Biological Sciences
Degree
Undergraduate
Mentor
Marcia M. Pierce
Mentor Department
Biological Sciences
Recommended Citation
Arthur, Sarah E.; Pierce, Marcia; and Donkin, Ryan, "Isolating Novel Phages Using Mycobacterium smegmatis" (2019). University Presentation Showcase Event. 9.
https://encompass.eku.edu/swps/2019/undergraduate/9
Abstract
Bacteriophages are viruses that infect bacterial hosts. Interest in bacteriophage research has grown as a future treatment option for human bacterial diseases, which could be used to avert the mounting crisis of antibiotic resistance. The presented project entitled Isolating Novel Phages Using Mycobacterium smegmatis aims to identify an individual phage possessing unique genetic information that can potentially be useful in further biomedical research. The process begins with the collection of a soil sample which is then enriched and used to create a filtrate. This filtrate was then purified through a series of dilutions, leading to the isolation of an individual phage. Once isolated, through the use of bioinformatics, the single phages genomic, physical, and molecular properties were deduced. This unique information is then characterized and placed into a singular database. This database can be utilized by researchers in current studies combating antibiotic resistance.
Presentation format
Poster
Isolating Novel Phages Using Mycobacterium smegmatis
Bacteriophages are viruses that infect bacterial hosts. Interest in bacteriophage research has grown as a future treatment option for human bacterial diseases, which could be used to avert the mounting crisis of antibiotic resistance. The presented project entitled Isolating Novel Phages Using Mycobacterium smegmatis aims to identify an individual phage possessing unique genetic information that can potentially be useful in further biomedical research. The process begins with the collection of a soil sample which is then enriched and used to create a filtrate. This filtrate was then purified through a series of dilutions, leading to the isolation of an individual phage. Once isolated, through the use of bioinformatics, the single phages genomic, physical, and molecular properties were deduced. This unique information is then characterized and placed into a singular database. This database can be utilized by researchers in current studies combating antibiotic resistance.
