University Presentation Showcase: Graduate Division
Assessing antibiotic resistance in Enterococci among roadkill wildlife and waterfowl scat in Kentucky.
Presenter Hometown
Richmond,Kentucky
Major
Environmental/occupational health and sustainability
Department
Environmental Health Science
Degree
Graduate
Mentor
JW Marion
Mentor Department
Environmental Health Science
Recommended Citation
Kosgei, Sharon C. Miss, "Assessing antibiotic resistance in Enterococci among roadkill wildlife and waterfowl scat in Kentucky." (2024). University Presentation Showcase Event. 12.
https://encompass.eku.edu/swps/2024/graduate/12
Abstract
Introduction: Antibiotic resistance (AR) is a global health threat. The study investigated wildlife, potential AR carriers, using roadkill and waterfowl scat swabs to assess AR.
Methods: In June 2023, 147 swabs were collected from 49 animals (36 roadkill, 13 waterfowl) for fecal bacteria assessment. Swabs were placed in Enterolert media with no antibiotic, ciprofloxacin-treated and tetracycline-treated, and field-incubated using a Hach portable incubator. Sixty-three presumed Enterococcus spp. isolates were strategically selected and inoculated on selective and general media for final characterization. Species identity and antibiotic susceptibility were determined using the Beckman Coulter MicroScan® autoScan-4 system with LabPro v4.42 and PC-44 panels.
Results: Enterococci growth rates varied among antibiotic treatments (98% no antibiotic, 69% tetracycline, 92% ciprofloxacin). Among 63 isolates, 25 (40%) showed resistance to tetracycline. Ciprofloxacin resistance was observed in 11 (17%), while vancomycin resistance was absent (0%). Daptomycin resistance and linezolid resistance were observed in seven (11%) and nine (14%) isolates, respectively. Recovery and identification were superior without antibiotics (100%) compared to tetracycline (71%) and ciprofloxacin (72%). Tetracycline-resistant isolates were prevalent in tetracycline treated media (p = 0.002).
Conclusion: The Enterolert method used is novel and confirms efficacy in isolating antibiotic-resistant Enterococci with or without antibiotics. Tetracycline resistance variations emphasize media formulations' role in surveillance precision. The results implicate wildlife as antibiotic resistance carriers, stressing the need for targeted investigations efforts to mitigate the spread of resistance between wildlife, the environment, livestock, and humans. These insights guide future strategies to reduce global antibiotic resistance through informed monitoring and interventions.
Presentation format
Poster
Assessing antibiotic resistance in Enterococci among roadkill wildlife and waterfowl scat in Kentucky.
Introduction: Antibiotic resistance (AR) is a global health threat. The study investigated wildlife, potential AR carriers, using roadkill and waterfowl scat swabs to assess AR.
Methods: In June 2023, 147 swabs were collected from 49 animals (36 roadkill, 13 waterfowl) for fecal bacteria assessment. Swabs were placed in Enterolert media with no antibiotic, ciprofloxacin-treated and tetracycline-treated, and field-incubated using a Hach portable incubator. Sixty-three presumed Enterococcus spp. isolates were strategically selected and inoculated on selective and general media for final characterization. Species identity and antibiotic susceptibility were determined using the Beckman Coulter MicroScan® autoScan-4 system with LabPro v4.42 and PC-44 panels.
Results: Enterococci growth rates varied among antibiotic treatments (98% no antibiotic, 69% tetracycline, 92% ciprofloxacin). Among 63 isolates, 25 (40%) showed resistance to tetracycline. Ciprofloxacin resistance was observed in 11 (17%), while vancomycin resistance was absent (0%). Daptomycin resistance and linezolid resistance were observed in seven (11%) and nine (14%) isolates, respectively. Recovery and identification were superior without antibiotics (100%) compared to tetracycline (71%) and ciprofloxacin (72%). Tetracycline-resistant isolates were prevalent in tetracycline treated media (p = 0.002).
Conclusion: The Enterolert method used is novel and confirms efficacy in isolating antibiotic-resistant Enterococci with or without antibiotics. Tetracycline resistance variations emphasize media formulations' role in surveillance precision. The results implicate wildlife as antibiotic resistance carriers, stressing the need for targeted investigations efforts to mitigate the spread of resistance between wildlife, the environment, livestock, and humans. These insights guide future strategies to reduce global antibiotic resistance through informed monitoring and interventions.
